Antioxidant and angiotensin I-converting enzyme (ACE) inhibitory peptides of rainbow trout (Oncorhynchus mykiss) viscera hydrolysates subjected to simulated gastrointestinal digestion and intestinal absorption

The objective of this work was to evaluate in vitro bioaccessibility, intestinal absorption, antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities peptides from rainbow trout viscera hydrolysate (H). Rainbow Viscera (V) hydrolyzed by Alcalase® 2.4L a degree hydrolysis (DH) 44.8 ± 2.5% achieved. its were subjected simulated gastrointestinal digestion (SGID) absorption across Caco-2/TC7 cell monolayers. After the with SGID V, species between 60.6 kDa 13.0 decreased, causing an increase less than 6.51 kDa. H did not modify oxygen radical absorbance capacity (ORAC) or ACE values but caused significant decrease hydroxyl (HORAC) (30.2%). It also produced ABTS cation (ABTS assay) scavenging activity ferric reducing power (FRAP) (9.46% 20.2%, respectively). Bioactive stable after they partially able cross monolayer, which demonstrates their possible potential act inside organism.